| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
A method to assess algicidal activity of microalgal extracts coupling microalgae produced in stirred closed photobioreactor operating in continuous with pulse amplitude modulated (PAM) fluorometry
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| Autores: |
Menguy, Eva
Dumontet, Vincent
Coulombier, Noémie
Meriot, Vincent
Le Dean, Loic
Barthelemy, Vanille
Jauffrais, Thierry
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| Data: |
2020
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| Ano: |
2020
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| Palavras-chave: |
Bioassay
Cytoxicity
Microalgae
Quantum efficiency
Fv/Fm
Non photochemical quenching
NPQ
Electron transport rate
Phytochemistry
Anti algal
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| Resumo: |
We describe in the present study a quick and reliable method based on chlorophyll a fluorescence to assess putative algicidal effect of different microalgal extracts. We couple microalgal production under chemostat cultivation mode to continuously produce a given microalgae species (e.g. Dunaliella salina in this study) at a stable physiological state to ease comparison between extracts tested; with a non-destructive method based on chlorophyll a fluorescence. Pulse amplitude modulated (PAM) fluorometry was used to assess over time the effect of different microalgal crude extracts on the efficiency of the photosystem II (PSII) of a tested microalgae (Dunaliella salina). • Microalgal production at stationary phase in stirred closed photobioreactor (PBR) operating in continuous have stable photophysiological parameters, which is a prerequisite to compare the impact of different algicidal compounds. • The combination of both methods, allows to quickly assess the algicidal effect of diverse microalgal (crude) extracts on the PSII efficiency of a tested microalgae. • The method may be used to identify and isolate algicidal molecules affecting algal PSII using a bio-guided isolation protocol.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00643/75524/76400.pdf
DOI:10.1016/j.mex.2020.101037
https://archimer.ifremer.fr/doc/00643/75524/
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| Editor: |
Elsevier BV
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| Formato: |
application/pdf
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| Fonte: |
Methodsx (22150161) (Elsevier BV), 2020 , Vol. 7 , P. 101037 (8p.)
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| Direitos: |
info:eu-repo/semantics/openAccess
restricted use
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